Protein Methods
  • Western blotting
  • BioID/TurboID
  • Immunofluorescence
  • Cell lysate preparation (RIPA Buffer)

Molecular Biology
  • Agarose electrophoresis of DNA
  • Restriction enzyme digestion of plasmids
  • NEBuilder assembly

Cell Culture
  • Mouse ES cell maintenance
  • Wnt medulloblastoma sphere cultures
  • Wnt medulloblastoma adherent cultures
  • Freezing mESCs
  • Freezing Wnt medulloblastoma cell lines

The Doble Lab

Basic Medical Sciences Building
University of Manitoba
Room 615-745 Bannatyne Avenue
Winnipeg, Manitoba
Canada, R3E 0J9

© Brad Doble 2022